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pSB700 lenti gRNA EYFP

Plasmid

#167906

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EBFP2

Plasmid

#167905

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2782

Plasmid

#160668

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Publication

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO2212

Plasmid

#141302

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO1212

Plasmid

#141299

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pB7WG_ASX

Plasmid

#98891

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Publication

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Publication

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Expression

Bacterial

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-FAK20

Plasmid

#59130

Purpose

Cloning vector for FAK fret sensor with 20 nm helix

Depositor

Sivaraj Sivaramakrishnan

Publication

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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HcRed1-C1

Plasmid

#54557

Purpose

Localization: C1 Cloning Vector, Excitation: 588, Emission: 618

Depositor

Michael Davidson

Publication

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

HcRed1

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

SYFP2

Expression

Bacterial

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQMD

Plasmid

#48103

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

myc tag

Expression

Bacterial

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Publication

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

HA tag

Expression

Bacterial

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

Plasmid

#201683

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Publication

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-CBh-eGFP

Plasmid

#206885

Purpose

cloning backbone for sgRNA using OptScf2

Depositor

Jacob Giehm Mikkelsen

Publication

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Type

Empty backbone

Promoter

U6

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE334

Plasmid

#153243

Purpose

M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDGE335

Plasmid

#153244

Purpose

M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDGE337

Plasmid

#153246

Purpose

M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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