We narrowed to 90,070 results for: Mal

1. pTBL681 CHYRON4 integration construct

   Plasmid

   #126449

   Purpose

   To integrate the CHYRON4 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6/3xLacO-CHYRON4 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6/3xLacO

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

   Plasmid

   #154779

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   LaG17-SynNotch
   

   Tags

   LaG17

   Expression

   Mammalian

   Mutation

   204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmirGLO-Calb2-3'UTR

   Plasmid

   #68363

   Purpose

   Luciferase miRNA Target Expression Vector

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   Calb2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   C to T change in UTR

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

4. Frt-V5-hspB3

   Plasmid

   #63104

   Purpose

   Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb3 (HSPB3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_UdgX-EE-UdgX-NG-nCas9

   Plasmid

   #163570

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW TetO NET1A-V5 WT

   Plasmid

   #69828

   Purpose

   Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
   

   Use

   Lentiviral

   Tags

   v5

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRT2-H2B-EGFP-V5-2A (IG157)

   Plasmid

   #99623

   Purpose

   To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   H2B-EGFP-V5
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmirGlo-CALB2-3'UTR mir-30 mut2

   Plasmid

   #74429

   Purpose

   Calb2 UTR with mutated mir-30 site 1 and 2

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   CALB2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mouse GFP-PRMT7 LDIG(69-72)AAAA

   Plasmid

   #65044

   Purpose

   Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

   Depositor

   Steven Clarke

   Article

   Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

   Insert

   PRMT7 (Prmt7 Mouse)
   

   Use

   Bacterial propagation

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L69A D70A I71A G72A

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pXPR_BRD003-sgRBFOX1-2098

    Plasmid

    #115452

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgRBFOX1-2098 (RBFOX1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_BRD003-sgRBFOX1-2083

    Plasmid

    #115451

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgRBFOX1-2083 (RBFOX1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

    Plasmid

    #136651

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:CNRZ_1066 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-super-retro puro RhoGDI2 shRNA

    Plasmid

    #58897

    Purpose

    produces an shRNA against RhoGDI2

    Depositor

    Channing Der

    Article

    Stevens et al Small GTPases. 2011 Jul;2(4):202-210. Ep

    Insert

    RhoGDI2 (ARHGDIB Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTBL649 CHYRON2 integration construct

    Plasmid

    #126446

    Purpose

    To integrate the CHYRON2 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON2 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG182 FGFR1 3'UTR mut

    Plasmid

    #12009

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N15 3'UTR mut (FGFR1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

16. CRY-GalVP16l (B694)

    Plasmid

    #92030

    Purpose

    expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    GalBD-VP16

    Expression

    Mammalian

    Mutation

    NLS sequence of CRY2 mutated

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. FRT3-Mb2-HA-mTfp1-2A (IG155)

    Plasmid

    #99621

    Purpose

    To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2-HA-mTfp1
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRT1-Mb2YFP-2A (IG153)

    Plasmid

    #99619

    Purpose

    To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2EYFP
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRT2-Mb2Tomato-2A (IG154)

    Plasmid

    #99620

    Purpose

    To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRT3-HA-H2B-Cerulean-2A (IG158))

    Plasmid

    #99624

    Purpose

    To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HA-H2B-Cerulean
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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