We narrowed to 3,866 results for: XIA

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ppyCAG_RNaseH1_D210N

Plasmid

#111904

Purpose

Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mHif1a

Plasmid

#242809

Purpose

Lentiviral vector expressing mouse Hif1a tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLCKOv3

Plasmid

#209046

Purpose

Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-SRSF1 siRNA-resitant

Plasmid

#218974

Purpose

Vector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

SRSF1 cDNA

Expression

Mammalian

Mutation

siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…

Promoter

CMV

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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LentiU6-LacZ-GFP-Puro (BB)

Plasmid

#170459

Purpose

For cloning gRNA block

Depositor

Yonglun Luo

Article

Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

Insert

hU6-BB-LacZ and EF1A-EGFP-2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 for gRNA and EF1A for EGFP-2A-Puro

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3b(1454/3172)P2P-mut

Plasmid

#21405

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

hypoxia response element mutated

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
FUGW-EGFP-mHif1a-T163A

Plasmid

#242810

Purpose

Lentiviral vector expressing mouse Hif1a-T163A mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T163A

Promoter

Ubiquitin C

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mHif1a-T163E

Plasmid

#242811

Purpose

Lentiviral vector expressing mouse Hif1a-T163E mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T163E

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-FL_WobbleMut

Plasmid

#209053

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WKKD

Plasmid

#111905

Purpose

Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-EGFP

Plasmid

#209059

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to EGFP

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

EGFP

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-opCas12a-6xNLS

Plasmid

#209022

Purpose

Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

opCas12a-6xNLS-Flag-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

FLAG and MYC

Expression

Mammalian

Mutation

E174R/S542R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-UltraCas12a-6xNLS

Plasmid

#209023

Purpose

Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

UltraCas12a-6xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

M537R/F870L

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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PET01-TAF5-ex8-Minigene-SRSF1 motif WT

Plasmid

#218972

Purpose

Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8

Expression

Mammalian

Promoter

RSV LTR

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-NoABE

Plasmid

#209043

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K164R)

Plasmid

#72555

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Tags

5XHRE

Expression

Mammalian

Mutation

K164R

Promoter

E1B minimal promoter

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-enCas12a-2xNLS

Plasmid

#209021

Purpose

Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

enCas12a-2xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A-pBabe-Hygro

Plasmid

#21676

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

cDNA changed amino acids from wt: Proline 405 t…

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K120R)

Plasmid

#72554

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Tags

5XHRE

Expression

Mammalian

Mutation

K120R

Promoter

E1B minimal promoter

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-zeo: alpha6mGFP

Plasmid

#35611

Purpose

mammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tag

Depositor

Henry Lester

Article

Xiao et al Biochem Pharmacol. 2011 Oct 15;82(8):852

Insert

nAChr alpha6mEGFP (Chrna6 Mouse)

Tags

mEGFP inserted after A405 with AGA linker on eith…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-TAF5-FL

Plasmid

#209051

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-EGFP

Plasmid

#209058

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged EGFP

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

EGFP

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-TAF5-FL

Plasmid

#209049

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-intergenic-As

Plasmid

#209026

Purpose

Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-CD46 Ex3_1-Lb

Plasmid

#209027

Purpose

Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-CD46 Ex3_2-Lb

Plasmid

#209028

Purpose

Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge