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Plasmid#133822PurposeEncodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationD363,365NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A D230,232N IRES GFP
Plasmid#133824PurposeEncodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationD230,232NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D303G F234C
Plasmid#170647PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD303G, C277A, F234CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D303G V304C
Plasmid#170648PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD303G, C277A, V304CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D365E F234C
Plasmid#170650PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD365E, C277A, F234CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D365E V304C
Plasmid#170651PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD365E, C277A, V304CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) I367T F234C
Plasmid#170653PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationI367T, C277A, F234CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) I367T V304C
Plasmid#170654PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationI367T, C277A, V304CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR S72A
Plasmid#179726PurposeLentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate releaseDepositorInsertiGluSnFR
UseLentiviralTagsExpressionMutationS72APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)
Viral Prep#198516-AAV5PurposeReady-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF-1aTagsmScarletAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV1)
Viral Prep#198516-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF-1aTagsmScarletAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-CCPG1
Plasmid#128262PurposeExpresses CCPG1 in mammalian cellsDepositorInsertCCPG1 (CCPG1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLS.402
Plasmid#187202PurposeExpresses retron Eco1 RTDepositorInsertRetron Eco1 Reverse Transcriptase
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermphRAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT003
Plasmid#171628Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-TRAC
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and EF1-aAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT009
Plasmid#171635PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-B2M
mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-gp78-FLAG
Plasmid#62370PurposeExpress C-terminally FLAG tagged gp78DepositorInsertgp78 (AMFR Human)
UseTagsFLAGExpressionMammalianMutation*PromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only