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We narrowed to 3,673 results for: PAM

Showing: 1061 - 1080 of 3673 results

  8. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

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  9. pECNUS2

    Plasmid
    #184885
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    184885-plasmid-map-sequence-id-435252

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  10. pECNUS3

    Plasmid
    #184886
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184886-plasmid-map-sequence-id-437894

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  11. pECNUS1

    Plasmid
    #184884
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184884-plasmid-map-sequence-id-437893

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  12. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  16. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  17. SpRYc

    Plasmid
    #175575
    Purpose
    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Article
    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:
    Insert
    SpRYc
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    PID of SpRY grafted onto the N-terminal domain of…
    Promoter
    CMV
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    175575-plasmid-map-sequence-id-345065

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  18. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  19. pYPQ166-SpRY

    Plasmid
    #161520
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9
    Use
    CRISPR; Gateway compatible zsprycas9 entry clone
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161520-plasmid-map-sequence-id-325640

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  20. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108860-plasmid-map-sequence-id-209932

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Showing: 1061 - 1080 of 3673 results