We narrowed to 1,827 results for: PORA

1. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

   Plasmid

   #12153

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Retroviral

   Tags

   6XHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. GEF-H1 FLARE191.pa

   Plasmid

   #134931

   Purpose

   GEF-H1 biosensor that has GEF activity.

   Depositor

   Klaus Hahn

   Article

   Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

   Insert

   GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
   

   Use

   Tags

   None.

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GEF-H1 FLARE212.pa

   Plasmid

   #134930

   Purpose

   GEF-H1 biosensor that does not have GEF activity.

   Depositor

   Klaus Hahn

   Article

   Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

   Insert

   GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
   

   Use

   Tags

   None.

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcpBpaRS_v2

   Plasmid

   #196485

   Purpose

   To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40(synthetase), Pol III tRNA promoter (tRNA), C…

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

   Plasmid

   #177265

   Purpose

   STREAMING-tag cassette

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

   Plasmid

   #164140

   Purpose

   AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

   Depositor

   Hirokazu Hirai, Hajime Hirase

   Article

   Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

   Insert

   R-CaMP1.07
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hGFAP

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_CAG_V5-LOV-Turbo-NES

   Plasmid

   #199670

   Purpose

   expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   AAV

   Tags

   NES and V5

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-GRAB_OT1.0

   Plasmid

   #185384

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. tet_pLKO.1_puro_shLuc

   Plasmid

   #136587

   Purpose

   Expresses an inducible short hairpin targeting firefly luciferase sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hGFAP-Pink Flamindo-WPRE-SV40

    Plasmid

    #178790

    Purpose

    AAV vector to express the red cAMP indicator in astrocytes

    Depositor

    Tetsuya Kitaguchi

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    Pink Flamindo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAL149

    Plasmid

    #22275

    Purpose

    Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB(1-908) (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mCherry-Kras4BCT (prenylation sequence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEVOL-NBK-1

    Plasmid

    #207639

    Purpose

    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The evolved synthetase NBK-1 contains mutations Y…

    Promoter

    araBAD

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mito-LOV-Turbo-V5

    Plasmid

    #199666

    Purpose

    expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    COX4I1 mito targeting sequence and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

    Plasmid

    #86194

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta Globin (HBB, Hbb Human, Mouse)
    

    Use

    Tet on

    Tags

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Tet on

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_eCBmut

    Plasmid

    #164605

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-AKAR4-NES

    Plasmid

    #64727

    Purpose

    Cytosol-targeted, FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

    Insert

    AKAR4-NES
    

    Use

    Tags

    Cerulean, NES, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcpAzpaRS_v2

    Plasmid

    #196483

    Purpose

    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174884

    Purpose

    Targeting vector for generating Sox2 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Sox2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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