We narrowed to 16,138 results for: Vars;

1. pTE3319

   Plasmid

   #107533

   Purpose

   Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RR)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K637R

   Promoter

   CMV

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE3316

   Plasmid

   #107532

   Purpose

   Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(RR)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K671R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE3317

   Plasmid

   #107531

   Purpose

   Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RVR)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K582V, N586R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE3179

   Plasmid

   #107528

   Purpose

   Expresses Mb crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and human U6

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-li-p33_RS-CLIP

   Plasmid

   #104634

   Purpose

   Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

   Depositor

   Sébastien Walchli

   Article

   Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

   Insert

   modified Invariant chain p33 (CD74 Human)
   

   Use

   Entry vector for gateway system

   Tags

   none

   Expression

   Mutation

   CLIP domain was replaced by 2 restriction sites

   Promoter

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE4497

   Plasmid

   #80339

   Purpose

   mammalian expression FnCpf1 nuclease and Fn crRNA

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   FnCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE4889

   Plasmid

   #88905

   Purpose

   Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(D917A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE4566

   Plasmid

   #88904

   Purpose

   Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   hMbCpf1(D986A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PH-PLCD1(R40L)-GFP

    Plasmid

    #51408

    Purpose

    Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 40 mutated to leucine

    Promoter

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC101_TIMER

    Plasmid

    #103057

    Purpose

    low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. RNA motif plasmid cloning backbone

    Plasmid

    #107253

    Purpose

    RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    RNA motif plasmid cloning backbone
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mRFP-FKBP12

    Plasmid

    #67514

    Purpose

    FKBP12 only

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12 (FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG5-ERRbeta2

    Plasmid

    #52186

    Purpose

    Expresses human ERRbeta2 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGR-1 TurboRFP

    Plasmid

    #52306

    Purpose

    Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Varma et al Lab Chip. 2015 Feb 4.

    Insert

    EGR-1 Promoter (Egr1 Mouse)
    

    Use

    Tags

    TurboRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7_hsa-miR-34a

    Plasmid

    #25791

    Depositor

    Judy Lieberman

    Article

    Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

    Insert

    hsa-miR-34a (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. PITPbeta-GFP

    Plasmid

    #58274

    Purpose

    mammalian expression of GFP-PITP beta

    Depositor

    Shamshad Cockcroft

    Article

    Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pAAV CAGG eGFP

    Plasmid

    #107707

    Purpose

    express eGFP in variety of cell types

    Depositor

    Troy Margrie

    Article

    Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21 E2F1

    Plasmid

    #90313

    Purpose

    Expresses human E2F1 in bacteria

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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