Addgene

We narrowed to 2,166 results for: ada.2

Showing: 1061 - 1080 of 2166 results

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pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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-111 hTF m2

Plasmid

#15449

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

All 3 Sp1 sites mutated

Promoter

Available since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only
pspCas9-ABE-3'UTR-sgRNA-2xMS2

Plasmid

#132554

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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-111 hTF m3

Plasmid

#15450

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

All 3 EGR-1 sites mutated

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
-111 hTF m4

Plasmid

#15451

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

All 3 Sp1 and EGR-1 sites mutated

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pNKX2-5-GFP-T2A

Plasmid

#203608

Purpose

Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-BE4max

Plasmid

#183679

Purpose

Expresses eNme2-C-BE4 in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

rAPOBEC1-neNme2-C-2xUGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-DNMT3a(R887E)-DNMT3l

Plasmid

#154141

Purpose

Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP

Use

Tags

HA and sfGFP

Expression

Mammalian

Mutation

R887E

Promoter

SFFV

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304 3xFlag-eRF1_IRES-GFP

Plasmid

#198393

Purpose

eRF1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eRF1 (ETF1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K107/113R-3xFlag_IRES-GFP

Plasmid

#198392

Purpose

RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site double mutant (K107/113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S197A

Plasmid

#131522

Purpose

Expresses MKP-3 with S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Expression

Mutation

changed Serine 197 to Alanine

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_J

Plasmid

#146709

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

Three non silent mutations N605S, K759M, G1999S a…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-F961A_R

Plasmid

#147386

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-F961A (gw Fly, Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_539-830_O

Plasmid

#147150

Purpose

Insect Expression of DmGW182_539-830

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_539-830 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_539-1115_O

Plasmid

#147151

Purpose

Insect Expression of DmGW182_539-1115

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_539-1115 (gw Fly)

Use

Tags

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-SD6Wmut_O

Plasmid

#147155

Purpose

Insect Expression of DmGW182-SD6Wmut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-SD6Wmut (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_604-1115_N

Plasmid

#147060

Purpose

Insect Expression of DmGW182_604-1115

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_604-1115 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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