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HybridA-IRES-tauLacZ LNL TV

Plasmid

#15651

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridA-IRES-tauLacZ LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
HybridB-IRES-tauLacZ LNL TV

Plasmid

#15652

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridB-IRES-tauLacZ LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
HybridD-IRES-tauGFP LNL TV

Plasmid

#15653

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridD-IRES-tauGFP LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
HybridG-IRES-tauGFP LNL TV

Plasmid

#15655

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridG-IRES-tauGFP LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
HybridI-IRES-tauGFP LNL TV

Plasmid

#15657

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridI-IRES-tauGFP LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pZNpF-deleted

Plasmid

#245372

Purpose

Negative control, luciferase cassette deleted

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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ISA585: PM-42

Plasmid

#241970

Purpose

42 kb phagemid.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

PM-42

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc_C

Plasmid

#146090

Purpose

Mammalian Expression of Rluc

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

Rluc

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p64T-XBMP2

Plasmid

#117253

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP2

Use

Xenopus expression

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSP64T-BMP4

Plasmid

#117254

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP4

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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Ternary BAC Reporter

Plasmid

#78220

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCyc-Cdc24

Plasmid

#66574

Purpose

Express an additional copy of Cdc24, integrated at TRP locus

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

Cdc24

Expression

Yeast

Promoter

pCyc1

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pUC120

Plasmid

#50350

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Type

Empty backbone

Use

Cloning vector

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pPS1494

Plasmid

#1377

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

pGAL-REV-GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

Plasmid

#239609

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1/7SKhybrid-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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3xFlag-eGFP-Flag-SETX

Plasmid

#218838

Purpose

Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

Depositor

Albert La Spada

Article

Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

Insert

Senataxin (SETX Human)

Tags

Flag and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239604

Purpose

Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENN236_CRISPRcharm_Kv2

Plasmid

#220839

Purpose

CRISPRcharm Kv2 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv2

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-ShuttleOnly

Plasmid

#171098

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Mutation

XbaI site added upstream of U6 promotor. GtoC mu…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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