We narrowed to 16,597 results for: puromycin

1. pPC1000

   Plasmid

   #174829

   Purpose

   Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Inserts

   CRISPRi SpCas9 sgRNA cassette
   PuroR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   EF1a and mouse U6

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-TetOne-Puro-PFDN2

   Plasmid

   #202664

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   prefoldin subunit 2 (PFDN2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDA_122

   Plasmid

   #208095

   Purpose

   Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

   Depositor

   John Doench

   Article

   Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRIPZ-bioGEF-SUMO2nc-PURO

   Plasmid

   #208043

   Purpose

   Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   SUMO2nc (SUMO2 Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Mutation

   Q90P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTBL680 CHYRON3 integration construct

   Plasmid

   #126448

   Purpose

   To integrate the CHYRON3 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6/3xLacO-CHYRON3 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6/3xLacO

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Promoter

   CMV immediate early

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-Flag-Polß(WT)-IRES-puro

   Plasmid

   #128653

   Purpose

   mammalian expression of Flag-Polß(WT) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO_HOXB13_#2

   Plasmid

   #70094

   Purpose

   Expression of shRNA to human HOXB13, puromycin selection

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLKO_HOXB13_#1

   Plasmid

   #70093

   Purpose

   Expression of shRNA to human HOXB13, puromycin selection

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. attB-mCh-Dnmt3b1-Poly(A)-PuroR

    Plasmid

    #65556

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-Flag-Polß(D256A)-IRES-puro

    Plasmid

    #128677

    Purpose

    mammalian expression of Flag-Polß(D256A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D256A

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR-v2-Blast-Puro

    Plasmid

    #167186

    Purpose

    Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-Flag-Avi-ires-CHD5

    Plasmid

    #68878

    Purpose

    Transient mammalian expression of CHD5 for BirA tagging

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG/AVI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pXPR_BRD003 sgATXN1L-1

    Plasmid

    #74970

    Purpose

    sgATXN1L-1, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTBL681 CHYRON4 integration construct

    Plasmid

    #126449

    Purpose

    To integrate the CHYRON4 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON4 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMCB320-sgZmat3.51.5

    Plasmid

    #169024

    Purpose

    Expresses an sgRNA targeting Zmat3

    Depositor

    Laura Attardi

    Article

    Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

    Insert

    sgRNA targeting Zmat3, mCherry, Puromycin resistance
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCB320-sgZmat3.55.9

    Plasmid

    #169026

    Purpose

    Expresses an sgRNA targeting Zmat3

    Depositor

    Laura Attardi

    Article

    Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

    Insert

    sgRNA targeting Zmat3, mCherry, Puromycin resistance
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid

    #163359

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

    Plasmid

    #163340

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF1-GRM3 (S610L)

    Plasmid

    #31800

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed serine 610 to leucine

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only