We narrowed to 29,297 results for: tat
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Plasmid#153445PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS
Plasmid#153444PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
B-HypaSpCas9
Plasmid#126763PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.DepositorInsertB-HypaSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN692A; M694A; Q695A; H698A; amino acids 1005-1013…PromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-B-eSpCas9
Plasmid#126772PurposeExpression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159AS197A
Plasmid#131523PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsa-cdk2 3'UTR sicheck2 mut
Plasmid#121124Purposeluciferase assayDepositorInserthsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
ExpressionBacterialMutationmiR-199 seed binding site mutatedAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2 199 mut
Plasmid#121122Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
ExpressionBacterialMutationmiR-199 seed binding site mutatedAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)
Plasmid#128383Purposeexpresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deletedDepositorInsertUbcH7 (UBE2L3 Human)
TagsBactrocera dorsalis parkin with mutation C463A an…ExpressionBacterialMutationmutation C86K in UbcH7 genePromoterlac promotorAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (G20V)
Plasmid#119308Purposelentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (C21Y)
Plasmid#119304Purposelentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)
Plasmid#119313Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (R39H)
Plasmid#119309Purposelentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cellsDepositorAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-I894A(pL84)
Plasmid#124404PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.DepositorInsertGal4-TET1-878-911(I894A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationI894AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-T898A(pL84)
Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationT898AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationL900AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L897A(pL84)
Plasmid#124401PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.DepositorInsertGal4-TET1-878-911(L897A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationL897AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)
Plasmid#119314Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cellsDepositorAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
gh130
Plasmid#106800Purposeexpression of gRNA targeting CSGALNACT2DepositorInsertCSGALNACT2 (CSGALNACT2 Human)
ExpressionMammalianAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15A
Plasmid#113011PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)DepositorInsertfull length tral (tral Fly)
ExpressionBacterialMutation15 PNG phosphorylation sites mutated to AAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF12
Plasmid#105593Purposeretrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertfull length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationsilent mutations to make it resistant to the targ…PromoterMSCV-LTRAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only