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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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B33

Plasmid

#74008

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B51

Plasmid

#74005

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B53

Plasmid

#74006

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B31

Plasmid

#74007

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Promoter

U6

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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B108-Cre2

Plasmid

#73990

Purpose

B108 enhancer of Blimp1 gene drives IRES-Cre expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108-IRES-Cre

Tags

Myc

Expression

Mammalian

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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Stagia3-Cre

Plasmid

#73991

Purpose

Cre in Stagia3 backbone; vector-Cre

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Cre

Tags

Myc

Expression

Mammalian

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-10X-tRNA

Plasmid

#70123

Purpose

Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Promoter

None – SUP4 pol III promoter provided by insert

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pLacI265-Wasabi

Plasmid

#86103

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI G265D

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

G265D

Promoter

T7A1O4/O3 and pLacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTetR89-Wasabi

Plasmid

#86104

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR A89D

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A89D

Promoter

ptetA and ptetR

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GST-Laforin C329S

Plasmid

#84682

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C278S

Plasmid

#84681

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C266S

Plasmid

#84680

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C250S

Plasmid

#84679

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C205S

Plasmid

#84678

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C169S

Plasmid

#84677

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin 2CS (C266S,C278S)

Plasmid

#84674

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pBN279

Plasmid

#86725

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phlh-8::FLP D5

Expression

Worm

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GST-Laforin 3CS (C250S,C266S,C278S)

Plasmid

#84676

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin 2CS_new (C278S, C329S)

Plasmid

#84675

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only