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pET:GACG:GFP

Plasmid

#60735

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM1276

Plasmid

#47986

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM1301

Plasmid

#47989

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH53388

Plasmid

#47990

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH41264

Plasmid

#47993

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM1251

Plasmid

#47995

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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R4-LexAp65-R3

Plasmid

#41438

Depositor

Steve Stowers

Insert

LexAp65

Expression

Bacterial

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pPROBE-GT'

Plasmid

#37815

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pOPTG-Tau-RING

Plasmid

#233691

Purpose

Expression of Tau-RING protein in bacteria

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

pOPTG

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSrRGECO1

Plasmid

#222940

Purpose

Expresses RGECO1 (fluorescent calcium indicator) and pac (puromycin resistance) in the Choanoflagellate, Salpingoeca rosetta

Depositor

Pawel Burkhardt

Article

Colgren et al Sci Adv. 2025 Jan 10;11(2):eadr7434. doi

Insert

RGECO1

Use

Salpingoeca rosetta expression

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG3

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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WTTEM1_pSTC0-zeo

Plasmid

#202351

Purpose

Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

TEM-1

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJM194A

Plasmid

#218124

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
luxAB

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-H1-Esp3I-mU6-Kan

Plasmid

#213015

Purpose

Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)

Depositor

Stephanie Roessler

Insert

H1 promoter, mU6 promoter, gRNA scaffolds

Promoter

H1 promoter

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-ccdB-6stop

Plasmid

#203511

Purpose

Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-ccdB-6stop

Plasmid

#203514

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-ccdB-6stop

Plasmid

#203512

Purpose

Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-1x-ccdB-6stop

Plasmid

#203519

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-2x-uA-ccdB-6stop

Plasmid

#203517

Purpose

Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Type

Empty backbone

Expression

Yeast

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge