We narrowed to 18,431 results for: multi
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Plasmid#227115PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0396DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene
ExpressionMammalianAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMVmin-BC0538-luc2
Plasmid#227140PurposeBarcoded assay, Ca2+ sensor; barcode BC05038DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
ExpressionMammalianAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176625PurposeTF A self-activation cassette in MultiFate-2.1DepositorInsertTRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
Plasmid#217511PurposeKD Recombinase dependent conditional smGdP-10XHA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
TagssmGdP-10XHAExpressionInsectAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GRE
Plasmid#172336PurposeGal4 Response elementDepositorPublicationInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept
Plasmid#182424PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteinsDepositorInsertsTagsVesicle Nucleating peptide (VNp), and Vesicle Nuc…ExpressionBacterialPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUb-Cas9-mCherry_cU6:6-sgRNA
Plasmid#190598PurposeThe plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA
UseCRISPRTagsmCherry - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10
Plasmid#180350Purposemammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10
Plasmid#180348Purposemammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianPromoterbidirectional TREAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184739Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2000V
Plasmid#183815PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2000VPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpG
Plasmid#179081PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationD1135L, S1136W, G1218K, E1219Q, R1335Q, T1337RPromoterCAGAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE xCas9
Plasmid#172652PurposeAll-in-one prime editor piggyBac transposon (xCas9)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertxCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)
Plasmid#169336PurposeExpression of the LT-Fucci(SA) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP
Plasmid#135562PurposeAn AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporterDepositorInsertsshRNA (mouse PKCd)
Nuc-EYFP
UseAAV and RNAiTags3xNLSExpressionMammalianPromoterCMV-IE and mU6Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
TagsC-terminal CAAX-box and mVenusExpressionMammalianPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
Plasmid#173865PurposeMammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
TagsC-terminal CAAX-box and mVenusExpressionMammalianPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)
Plasmid#160628PurposeModule for the constitutive expression of the nptII, Cas9 and DsRed genes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
UseCRISPRMutationBsaI and BsmBI sites removedPromoterPnos, 35S, 35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir144 EF1Alpha-puro-T2A-BFP
Plasmid#164791PurposeExpress miR-144 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralExpressionMammalianPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only