We narrowed to 15,822 results for: LEA

1. pHB-2

   Plasmid

   #140955

   Purpose

   For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R238S mutation in PhaC

   Promoter

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF mito-cGFP (matrix)

   Plasmid

   #188904

   Purpose

   Expresses mitochondrial GFP

   Depositor

   Edwin Chapman

   Article

   Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

   Insert

   mitochondrial GFP
   

   Use

   Tags

   Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. JAK3 (JAK3A-c023)

   Plasmid

   #98229

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JAK3A (JAK3 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Expression

   Mutation

   pfam00069:Pkinase 822-1091

   Promoter

   polyhedrin

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. KEAP1 (KEAP1A-c029)

   Plasmid

   #110247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_449

   Plasmid

   #179093

   Purpose

   nuclease Cas expression

   Depositor

   David Root, John Doench

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   SpG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-puro-NLS-LSSmKate2

   Plasmid

   #34586

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS-LSS-mKate2
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

   Promoter

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAD6

   Plasmid

   #134349

   Purpose

   E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

   Depositor

   Seth Darst

   Article

   Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

   Insert

   mfd (mfd E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-iGlu3Fast

   Plasmid

   #226500

   Purpose

   Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

   Depositor

   Katalin Torok

   Article

   Tran et al bioRxiv, August 30th, 2024

   Insert

   iGluSnFR3 S72T
   

   Use

   Tags

   IgK, Myc tag, and PDGFR Beta TM domain

   Expression

   Mammalian

   Mutation

   S72T

   Promoter

   CMV

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECFP-DEVG-Venus

   Plasmid

   #24538

   Depositor

   Peter Sorger

   Article

   Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

   Insert

   ECFP-DEVG-Venus
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   DEVDR mutated to DEVG

   Promoter

   CMV

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET26b_Nb.AT110

    Plasmid

    #135480

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA-FRT-FAK30

    Plasmid

    #59121

    Purpose

    FAK fret sensor with 30 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PKCalpha.FLAG

    Plasmid

    #10805

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D332G-please see comments below

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. JMJD1CA-c089

    Plasmid

    #79644

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD1C (JMJD1C Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH220

    Plasmid

    #122836

    Purpose

    dCas9 with GreenGate B-C flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Contains D10A and H840A mutations

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. NES-iRFP-P4Mx1

    Plasmid

    #108144

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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