We narrowed to 15,044 results for: invs

1. pCR3-FLAG-Gephyrin GC

   Plasmid

   #68818

   Purpose

   Flag tagged mammalian expression of GC domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

   Plasmid

   #181843

   Purpose

   GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of residues 12-112 in PKA RIα.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

   Plasmid

   #113953

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S, S212N, and …

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO myc Separase Non-Cleavable

   Plasmid

   #59826

   Purpose

   Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_DIO_R106W-HaloTag

   Plasmid

   #164063

   Purpose

   Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

   Depositor

   Nathaniel Heintz

   Article

   Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

   Insert

   MeCP2 R106W (Mecp2 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

   Plasmid

   #121814

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-2B-Gata4

   Plasmid

   #98615

   Purpose

   Gateway entry vector for Gata4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Gata4 (Gata4 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+ Flag-RAGB99L

   Plasmid

   #128101

   Purpose

   Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Ras related GTP binding B (RRAGB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q99L (CAA to CTA)

   Promoter

   CMV

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST53-LRRK2-K1347A

   Plasmid

   #25052

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 1347 mutated to Alanine

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Promoter

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pKK-NoTag

    Plasmid

    #105767

    Purpose

    Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EPHA3_1-972 pcDNA3.1

    Plasmid

    #102739

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA-hIRF-1 #12

    Plasmid

    #61079

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    gRNA_hIRF1 promoter #12 (IRF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Opto-GPR12

    Plasmid

    #106003

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR12; Opto-GPR12; A5)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR12 (GPR12 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid

    #121841

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid

    #168099

    Purpose

    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nUbc-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Cx43-YA/VD-GFP

    Plasmid

    #60703

    Purpose

    mammalian expression of rat Cx43 YA/VD mutant fused to GFP

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only