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pNCMV HPV8 E6 K136N

Plasmid

#87657

Purpose

Flag/HA tagged HPV8 E6 K136N

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

K136N

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K142A

Plasmid

#87660

Purpose

Flag/HA tagged HPV8 E6 K142A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

K142A

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 L59D

Plasmid

#87655

Purpose

Flag/HA tagged HPV8 E6 L59D

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

L59D

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV MmuPV1 E6 R130A

Plasmid

#87654

Purpose

Flag/HA tagged MmuPV1 E6 R130A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MmuPV1 E6

Tags

Flag and HA

Expression

Mammalian

Mutation

R130A

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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mCm7RNA6A

Plasmid

#84367

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2 m7-gRNA6

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
PHBA03

Plasmid

#83537

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG MKRN1 S109D

Plasmid

#78775

Purpose

To overexpress MKRN1 S109D in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S109D_mutation

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-zNanogHD-pgk-hph

Plasmid

#74910

Purpose

Mammalian expression of zNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJK359

Plasmid

#71570

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-TEF2

Plasmid

#65340

Purpose

construction of TEF2 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 terminator

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TDH3

Plasmid

#65336

Purpose

construction of TDH3 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3 promoter

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-ADH1

Plasmid

#65339

Purpose

construction of ADH1 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ADH1 terminator

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTD125 GFP:POP1

Plasmid

#71387

Purpose

localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

POP1 (POP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
pJB051 (mEos2-ZapA)

Plasmid

#72650

Purpose

Inducible expression of mEos2-ZapA in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

mEos2-ZapA

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJK591

Plasmid

#71589

Purpose

Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Mutation

changes threonine-2 to alanine and aspartate-177 …

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK286

Plasmid

#71567

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

formyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_FG

Plasmid

#66070

Purpose

MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_GH

Plasmid

#66071

Purpose

MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK358

Plasmid

#71569

Purpose

Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

putative CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only