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pAc5.1B-EGFP-DmPABPC1-V5His6_G

Plasmid

#146352

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Sticky-173a-16X

Plasmid

#165453

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK108

Plasmid

#173732

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

MP

Use

Other

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK259

Plasmid

#173752

Purpose

fluorescent par-6(delPB1) expression

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR

Expression

Worm

Mutation

delPB1

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-171a-16X

Plasmid

#165452

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-nonpalindromic-169an-4X

Plasmid

#165413

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-167a-16X

Plasmid

#164494

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-169a-16X

Plasmid

#164495

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0173

Plasmid

#154640

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

35s (ΔAP2+1) Firefly luciferase expression cassette [35s (ΔAP2+1)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFVGe2

Plasmid

#149500

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, GmR, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-GmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBLlCa5

Plasmid

#149501

Purpose

Broad-Host range expression vector, pBBR origin, LacI repressor, PLlacO-1 promoter, AmpR, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-AmpR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKPcR5

Plasmid

#149467

Purpose

Broad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

PcaU(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P3B5B

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKVR5

Plasmid

#149469

Purpose

Broad-Host range expression vector, RK2 and pUC origin, VanR(AM) repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0030

Plasmid

#162312

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0258

Plasmid

#162291

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0025

Plasmid

#162283

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0512

Plasmid

#154751

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_057 Firefly luciferase expression cassette [MinSyn_057::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0513

Plasmid

#154752

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_062 Firefly luciferase expression cassette [MinSyn_062::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0483

Plasmid

#154730

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_115 Firefly luciferase expression cassette [MinSyn_115::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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