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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,901 results for: cloning

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Showing: 10961 - 10980 of 63901 results

  1. mGeos2-VESS-N1

    Plasmid
    #54589
    Purpose
    Localization: N1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mGeos2-VESS
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. wtEos-N1

    Plasmid
    #54681
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    wtEos
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. wtKikGR-C1

    Plasmid
    #54547
    Purpose
    Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    wtKikGR
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pBR HPV31 9E

    Plasmid
    #221009
    Purpose
    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322
    Depositor
    Alison McBride
    Insert
    Human papillomavirus type 31 genome 9E isolate
    Use
    Cloning vector
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    221009-plasmid-map-sequence-id-436075

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  10. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  14. XW17

    Plasmid
    #65835
    Purpose
    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)
    Depositor
    Kang Shen
    Publication
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Tags
    mCherry
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65835-plasmid-map-sequence-id-120565

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  15. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    none
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

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  16. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Publication
    Pulgarin et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Expression
    Mammalian
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    220498-plasmid-map-sequence-id-442816

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Showing: 10961 - 10980 of 63901 results