We narrowed to 4,038 results for: Aes

1. pDONR221-MICU1-sh1-rORF

   Plasmid

   #31764

   Depositor

   Vamsi Mootha

   Article

   Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

   Insert

   MICU1 (MICU1 Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   RNAi-resistant ORF (rORF). 8 synonymous changes i…

   Promoter

   None

   Available since

   May 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-GLT1a-V5

   Plasmid

   #162517

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1a (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-GLT1b-V5

   Plasmid

   #162518

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1b (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

   Plasmid

   #121835

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myopathy Loop Mutant Nuclear Myosin I YFP

   Plasmid

   #60243

   Purpose

   Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   Deleted I339, I340, G343, E344, E345,L346

   Promoter

   CMV

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

   Plasmid

   #121824

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/LSD1 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p7612 MSCV-P C-FlagHA 16E7 E10K

   Plasmid

   #163303

   Purpose

   Expresses HPV16 E7 E10K

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   HPV16 E7 E10K
   

   Use

   Retroviral

   Tags

   FlagHA

   Expression

   Mutation

   E10K

   Promoter

   MSCV LTR

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

   Plasmid

   #109318

   Purpose

   Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   SaCas9 RuvC gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

   Plasmid

   #109317

   Purpose

   Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-mqgDUX4mqg*

    Plasmid

    #21177

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 6163 (numbering acco…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

11. IX301: pMVP (L3-L2) myc epitope tag + polyA

    Plasmid

    #121753

    Purpose

    pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    myc epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDP1.2

    Plasmid

    #171092

    Purpose

    Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
    

    Use

    Synthetic Biology

    Tags

    Emerald, Geminin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid

    #121751

    Purpose

    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FLAG epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. KZ401: pMVP (L3-L2) P2A-Neo + polyA

    Plasmid

    #121781

    Purpose

    pMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Neo + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KZ601: pMVP (L3-L2) P2A-Zeo + polyA

    Plasmid

    #121783

    Purpose

    pMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Zeo + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KZ901: pMVP (L3-L2) P2A-eGFP + WPRE

    Plasmid

    #121772

    Purpose

    pMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eGFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid

    #121834

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-Cas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LA601: pMVP (L3-L2) P2A-Puro + WPRE

    Plasmid

    #121785

    Purpose

    pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Puro + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KZ501: pMVP (L3-L2) P2A-Puro + polyA

    Plasmid

    #121780

    Purpose

    pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Puro + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

    Plasmid

    #121830

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/KRAB (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge