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Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorUseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
aMHC-mCherry-Rex-Blasticidin
Plasmid#21228DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceJune 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4511NES
Plasmid#138375PurposeBooster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.DepositorInsertBooster-ERK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4493NES
Plasmid#138373PurposeBooster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.DepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
aMHC-eGFP-Rex-Neo
Plasmid#21229DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceJune 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre-T2A-mRuby2
Plasmid#102989PurposeExpresses Cre and mRuby2DepositorInsertsCre (site-specific recombinase)
mRuby2
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4580NES
Plasmid#138376PurposeBooster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.DepositorInsertBooster-JNK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
UseTagsExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4591NES
Plasmid#138377PurposeBooster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.DepositorInsertBooster-ROCK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Caveolin-C-10
Plasmid#55468PurposeLocalization: Membrane, Excitation: 462, Emission: 492DepositorInsertNM_001753 (CAV1 Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
aMHC-puro rex-neo
Plasmid#21230DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSW003-PpsbA-mNeonGreen
Plasmid#205019PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPpsbAAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
aMHC-Puro Rex-Blast
Plasmid#21231DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only