Skip to main content
Addgene

We narrowed to 1,166 results for: ncl

Showing: 1081 - 1100 of 1166 results
  1. MKC84_HBB(HBA1reg-HBA1)

    Plasmid
    #232410
    Purpose
    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.
    Depositor
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. MKC83_HBB(HBBreg-HBA1)

    Plasmid
    #232409
    Purpose
    AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.
    Depositor
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid
    #194195
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid
    #194196
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid
    #194197
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 449)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid
    #194191
    Purpose
    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid
    #194192
    Purpose
    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    3 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pGL3-pSMANTIS_1kb_KLF2mut 548

    Plasmid
    #194194
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. SIN40C.EFS.eGFP.mArid3a-3'UTR-WT

    Plasmid
    #169299
    Purpose
    Constitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Insert
    Arid3a-3'UTR-WT (Arid3a Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. SIN40C.EFS.eGFP.ARID3A-3'UTR-WT

    Plasmid
    #169297
    Purpose
    Constitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Insert
    ARID3A-3'UTR-WT (ARID3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. MKC62_HBB(HBA1reg-HBA1-2A-YFP)

    Plasmid
    #232408
    Purpose
    AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.
    Depositor
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Insert
    DmelOrco (Orco Fly)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1081 - 1100 of 1166 results