We narrowed to 15,981 results for: LEA

1. pUE1-TSP-UL32

   Plasmid

   #162658

   Purpose

   Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   UL32
   

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG476

   Plasmid

   #40198

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (S404A) (mex-5 Nematode)
   

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   S404A

   Promoter

   pie-1

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

   Plasmid

   #154399

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   SARS-CoV2 N Protein (N SARS-CoV2)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-BFP-LaminB1

   Plasmid

   #184460

   Purpose

   Nuclear membrane-targeted EBFP2 expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   EBFP2-LaminB1
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRATIO2214

   Plasmid

   #141303

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   mCerulean and mScarlet-I

   Expression

   Plant

   Promoter

   UBQ10 promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP

   Plasmid

   #179646

   Purpose

   TVMVP-inducible IL-12 RELEASE construct

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   IL12 A/B
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. hOtub1

   Plasmid

   #26959

   Depositor

   Cynthia Wolberger

   Article

   Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

   Insert

   Otub1 (OTUB1 Human)
   

   Tags

   His6 (TEV cleavable)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. [GG][Pin]

   Plasmid

   #97191

   Purpose

   Pin nuclease domain with Pum cloning site

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Insert

   Pin domain
   

   Use

   Synthetic Biology

   Tags

   Pum cloning site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_450

    Plasmid

    #179094

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRATIO4214

    Plasmid

    #141314

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mCerulean and mScarlet-I

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEXC3GH (Hyg)

    Plasmid

    #110082

    Purpose

    Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. csr-1_son-1_iRFP670

    Plasmid

    #191749

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Tags

    NCU04502 promoter and iRFP670

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PA-mCherry1-LaminA-C-18

    Plasmid

    #57185

    Purpose

    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    LaminA (LMNA Human)
    

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMEXC3GH (Kan)

    Plasmid

    #110081

    Purpose

    Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PsaA

    Plasmid

    #159997

    Purpose

    Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    PsaA (19-309)
    

    Tags

    His6-tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA-FRT-FAK20

    Plasmid

    #59120

    Purpose

    FAK fret sensor with 20 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSETA_crE6G2

    Plasmid

    #172929

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6G2
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Val317Gly, Met316Gly

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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