We narrowed to 16,144 results for: LEA
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Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialPromoterT7lacAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c012
Plasmid#173099PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c010
Plasmid#173100PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad-UGAcS
Plasmid#172137Purposebacteria expression plasmid for a UDP-GlcNAc sensorDepositorInsertUGAcS
ExpressionBacterialMutationH605Q- please see depositor commentsPromoteraraBADAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-H2B_Halo_T2A_EGFP
Plasmid#135444PurposeMamalian cell expression of nuclear HaloTag7 and cytosolic EGFPDepositorInsertH2B-HaloTag7 and EGFP
TagsH2BExpressionMammalianPromoterCMV/TetOAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-H-Lock
Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZY101
Plasmid#73932PurposeThis binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.htmlDepositorInsertbar
ExpressionPlantPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::Halo7
Plasmid#87648PurposeFor creating drosophila transgenics expressing UAS-Syt::Halo7DepositorInsertSyt::Halo7
ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-jGCaMP7b-XN
Plasmid#178362PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.DepositorInsertjGCaMP7b-XN
ExpressionMammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS253
Plasmid#114271PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxMutation*please see depositor comment belowAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
BRD7 (BRD7A-c005)
Plasmid#98245PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRD7A (BRD7 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00439:Bromodomain 136-223PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
NES-mTagBFP2-P4Mx1
Plasmid#108142PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
ExpressionMammalianPromoterCMVAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only