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We narrowed to 63,067 results for: cloning

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Showing: 11061 - 11080 of 63067 results

  2. psLIB U6

    Plasmid
    #229956
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229956-plasmid-map-sequence-id-461573

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  3. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

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  4. psLIB TRE

    Plasmid
    #229955
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229955-plasmid-map-sequence-id-461253

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  5. pCm N-term MBP SMT3 NikJC199U

    Plasmid
    #174363
    Purpose
    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Publication
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Tags
    8xHis, MBP, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174363-plasmid-map-sequence-id-342843

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  6. pCm N-term MBP NikJC199U

    Plasmid
    #174364
    Purpose
    Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Publication
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174364-plasmid-map-sequence-id-342851

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Showing: 11061 - 11080 of 63067 results