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pLBS.EF

Plasmid

#129335

Purpose

Lentivirus vector

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

unspecified

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pX131

Plasmid

#167149

Purpose

MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

4'-phosphopantetheinyl transferase, npgA

Use

Synthetic Biology

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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MTK8a_004

Plasmid

#123888

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8a_005

Plasmid

#123889

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8_004

Plasmid

#123884

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK7_011

Plasmid

#123878

Purpose

Encodes the 5' 500 bp hThal4.10-2-gh18 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hTha5.10-2-hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_014

Plasmid

#123881

Purpose

Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3' HIV LTR (3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_019

Plasmid

#123832

Purpose

Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d2PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_026

Plasmid

#123718

Purpose

Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSFFVc

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_025

Plasmid

#123687

Purpose

Encodes ConL3 and human D1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-D1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_026

Plasmid

#123688

Purpose

Encodes ConL4 and human E1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-E1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_030

Plasmid

#123692

Purpose

Encodes ConL1 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_010

Plasmid

#123673

Purpose

Encodes ConL2 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_011

Plasmid

#123674

Purpose

Encodes ConL3 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_014

Plasmid

#123677

Purpose

Encodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS'-FRT

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GGE209

Plasmid

#120793

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsUp-Mfe

Use

Cloning/assembly plasmid

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GGE104

Plasmid

#120744

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-3AB)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_EYK300-3AB

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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