We narrowed to 25,058 results for: Nes

1. SpCas9-mSA*

   Plasmid

   #182952

   Purpose

   Express SpCas9-mSA*

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   SpCas9-mSA*
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAp63gamma-(3X)HA

   Plasmid

   #26977

   Depositor

   David Sidransky

   Article

   Upadhyay et al Biochem Biophys Res Commun. 2007 May 18.

   Insert

   TAp63gamma (TP63 Human)
   

   Use

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRB-mCherry-PLIN3

   Plasmid

   #222420

   Purpose

   To express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   PLIN3 (PLIN3 Human)
   

   Use

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDA007

   Plasmid

   #131380

   Purpose

   ORFeus expression, WT

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   ORFeus-Hs
   

   Use

   Tags

   ORF2-3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. cr3Dd

   Plasmid

   #184862

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJEP207-AAV-CMV-MCS-pA

   Plasmid

   #78318

   Purpose

   AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-Akt2(WT)

   Plasmid

   #86623

   Purpose

   Expresses mCherry-tagged human Akt2(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2 (AKT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD428

    Plasmid

    #202081

    Purpose

    Cas9/sgRNA plasmid targeting the C-terminus of Myh9

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PylRS-AS

    Plasmid

    #137908

    Purpose

    For incorporation of fluorophenylalanine derivatives

    Depositor

    Wenshe Liu

    Article

    Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Containing N346A, C348S mutations

    Promoter

    pBAD

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAV1-mEGFP

    Plasmid

    #27704

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS4333 yeTadA1.0-T7 RNAP

    Plasmid

    #137735

    Purpose

    pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

    Depositor

    Christina Smolke

    Article

    Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

    Insert

    yeTadA1.0-T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    E366G (See depositor comment below)

    Promoter

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE TRE dCas9

    Plasmid

    #50915

    Purpose

    Tet-regulatable dCas9 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-C1-CAMKIIbeta

    Plasmid

    #21227

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIbeta (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0002.

    Promoter

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-DAG1

    Plasmid

    #205149

    Purpose

    Overexpress DAG1

    Depositor

    Monkol Lek

    Insert

    Dystroglycan 1 (DAG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Carries common missense variant c.41C>G (p.Ser…

    Promoter

    EF-1a

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. acta1-mRuby2-dGBP

    Plasmid

    #163855

    Purpose

    Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    dVHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV2_HLF-ZE

    Plasmid

    #175034

    Purpose

    HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

    Depositor

    Guy Sauvageau

    Article

    Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

    Insert

    HLF ires ZsGreen P2A tEGFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIIS638

    Plasmid

    #238210

    Purpose

    Expresses native SpTasH TIGR locus. CmR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    SpTasH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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