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pU6-Fn-crRNA

Plasmid

#78958

Purpose

Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-GFP-IRES-AP

Plasmid

#18807

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-GFP-IRES-AP

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBSMrnaStrp

Plasmid

#67508

Purpose

Express the tRNAMet scaffold with the streptavidin aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with streptavidin aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-1HA

Plasmid

#18027

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1 (Wnt1 Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP -R

Plasmid

#48887

Purpose

Positive feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

sfGFP and luxI

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP

Plasmid

#18811

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Chx10 (164bp crit)+SV40p-tdTomato-IRES-AP

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pCZGY2139

Plasmid

#74111

Purpose

Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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Cas9m2

Plasmid

#47314

Purpose

Cas9 D10A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Cabp5 (445bp crit)-tdTomato-IRES-AP

Plasmid

#18812

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Cabp5 (445bp crit)-tdTomato-IRES-AP

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pCS2+ MT Tcf3 (Xenopus)

Plasmid

#13439

Depositor

Marc Kirschner

Article

Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

Insert

Tcf3 (tcf7l1.S Frog)

Tags

Myc Tag

Expression

Mammalian

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
LP580

Plasmid

#90205

Purpose

pCMV-myc with cDNA encoding HsTRAPPC13 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC13 (TRAPPC13 Human)

Tags

Myc

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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DMPK

Plasmid

#38892

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DMPK (DMPK Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-Gm-REP

Plasmid

#64966

Purpose

mini-Tn7 vector with oriR6K, for attTn7 site determination

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

oriR6K

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFH47

Plasmid

#125812

Purpose

Level0 LbCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-51

Plasmid

#79966

Purpose

Late1-O3-33-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-AviTag

Plasmid

#67175

Purpose

CRISPaint universal donor for AviTag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

AviTag

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-09

Plasmid

#79924

Purpose

Palm3-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-09

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pALPS eGFP

Plasmid

#101323

Purpose

Encodes eGFP

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

eGFP

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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