We narrowed to 24,685 results for: Hal

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pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41_HaloTag9

Plasmid

#169331

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41_HaloTag9

Plasmid

#169331

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E

Plasmid

#175027

Purpose

Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E

Plasmid

#175027

Purpose

Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B R108E

Plasmid

#176004

Purpose

Expresses WIPI2B (R108E) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (R108E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Arginine 108 to Glutamate

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B R108E

Plasmid

#176004

Purpose

Expresses WIPI2B (R108E) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (R108E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Arginine 108 to Glutamate

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBSc polyA + Shal2

Plasmid

#16144

Depositor

Larry Salkoff

Article

Butler et al Science. 1989 Feb 17. 243(4893):943-7.

Insert

Shal (Shal Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pBSc polyA + Shal2

Plasmid

#16144

Depositor

Larry Salkoff

Article

Butler et al Science. 1989 Feb 17. 243(4893):943-7.

Insert

Shal (Shal Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HaloLICNot

Plasmid

#140185

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Halo

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HaloLICNot

Plasmid

#140185

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Halo

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-HaloTagx2

Plasmid

#140150

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

GGA2

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-HaloTagx2

Plasmid

#140150

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

GGA2

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:HaloTag

Plasmid

#179785

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG::HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:HaloTag

Plasmid

#179785

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG::HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge