We narrowed to 4,009 results for: MEA

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Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2

Plasmid

#69624

Purpose

A FRET sensor for Galphai2 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCH_CREB1

Plasmid

#64938

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CREB1 Homology Arms (CREB1 Human)

Tags

3X Flag P2A Neo

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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Gluc-LHCGR

Plasmid

#221781

Purpose

Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

Depositor

Adolfo Rivero-Muller

Article

Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

Insert

LHCGR (LHCGR Human)

Tags

Gaussia Luciferase and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

Plasmid

#69623

Purpose

A FRET sensor for Galphai1 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

Plasmid

#69625

Purpose

A FRET sensor for Galphai3 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Inserts

Gbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2

Tags

ciruclar permutated Venus (cp173V) and mTurquoise…

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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PX458_KMT2B_2

Plasmid

#101075

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KMT2B_1

Plasmid

#101074

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521K-peGFP-N1

Plasmid

#228588

Purpose

Expresses TMEM16F-I521K-peGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I521K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Human a3 nicotinic receptor subunit EM construct

Plasmid

#163960

Purpose

pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

Depositor

Ryan Hibbs

Article

Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

Insert

Human alpha3 nicotinic acetylcholine receptor subunit EM construct

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

apocytochrome b(562)RIL (BRIL)

Expression

Mammalian

Mutation

Asn348-Ser402 replaced with apocytochrome b(562)R…

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I611K-peGFP-N1

Plasmid

#228581

Purpose

Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I611K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPBC-LG3-tdT

Plasmid

#62810

Purpose

pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AA Clover

Plasmid

#90238

Purpose

Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AA mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Tags

14xHis on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1950

Plasmid

#205983

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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