We narrowed to 3,318 results for: cat.3

1. pKESK22

   Plasmid

   #64857

   Purpose

   expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

   Depositor

   Karin Schnetz

   Article

   Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GG-dest

   Plasmid

   #69538

   Purpose

   Destination plasmid for gRNA concatenation Golden Gate cloning

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Type

   Empty backbone

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-pHoran4

   Plasmid

   #61558

   Purpose

   Orange fluorescent protein based pH indicator in bacterial expression vector

   Depositor

   Robert Campbell

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   pHoran4
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET-6xHis-mCherry(-6)-L1

   Plasmid

   #205046

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   mCherry(-6)-L1
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-mCherry(-6)-L2

   Plasmid

   #205047

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   mCherry(-6)-L2
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-6xHis-GFP(-6)-L1

   Plasmid

   #205027

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-L1
   

   Tags

   KGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-6xHis-GFP(-6)-L2

   Plasmid

   #205028

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-L2
   

   Tags

   KGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-6xHis-GFP(-6)-H1

   Plasmid

   #205030

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H1
   

   Tags

   KKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-GFP(-6)-H2

   Plasmid

   #205031

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2
   

   Tags

   KKRRRKKKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-PUb-SSA

    Plasmid

    #52910

    Purpose

    Ae aegypti PUb promoter Single Stranded Annealing reporter

    Depositor

    Zach Adelman

    Article

    Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

    Insert

    Luciferase duplication-TALEN recognition sites
    

    Use

    Luciferase

    Promoter

    Ae aegypti polyubiquitin

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. piGECI-NES-N1

    Plasmid

    #160422

    Purpose

    Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Flag-Tet1 CD

    Plasmid

    #70129

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain.

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Flag-Tet2 CD

    Plasmid

    #72219

    Purpose

    Express flag-tagged mouse Tet2 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 2 (Tet2 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET2 catalytic domain.

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CaMKII-iGECI

    Plasmid

    #160423

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid

    #70666

    Purpose

    Reduced the expression of gamma-tubulin in murine cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    

    Use

    U6 promoter

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2059

    Plasmid

    #143997

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-3 (NKX2-3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKII-iGECI-NES

    Plasmid

    #160424

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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