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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,378 results for: halo

Showing: 1101 - 1120 of 1378 results

  2. pHIs8-4b-SaDAH::G226D

    Plasmid
    #140130
    Purpose
    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli
    Depositor
    Jing-Ke Weng
    Article
    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:
    Insert
    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    Use
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    changed Gly226 to Asp226
    Promoter
    T7
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140130-plasmid-map-sequence-id-275079

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  3. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PhaR promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174383-plasmid-map-sequence-id-343840

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  4. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  6. hSyn-GEM

    Plasmid
    #187648
    Purpose
    Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.
    Depositor
    Erin O'Shea
    Article
    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75
    Insert
    PfV
    Use
    AAV
    Tags
    HaloTag and SNAP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    187648-plasmid-map-sequence-id-369068

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  8. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  9. pETDuet-solo-NHis6-NocO-H286A

    Plasmid
    #240409
    Purpose
    expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240409-plasmid-map-sequence-id-478125

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  10. pETDuet-solo-NHis6-NocO-H132A

    Plasmid
    #240404
    Purpose
    expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240404-plasmid-map-sequence-id-478123

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  11. pETDuet-solo-NHis6-NocO-E129A

    Plasmid
    #240403
    Purpose
    expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240403-plasmid-map-sequence-id-478122

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  12. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid
    #240402
    Purpose
    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240402-plasmid-map-sequence-id-478121

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  13. pETDuet-solo-NHis6-NocO-E252A

    Plasmid
    #240405
    Purpose
    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240405-plasmid-map-sequence-id-478119

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  14. pETDuet-solo-NHis6-NocO-E283A

    Plasmid
    #240408
    Purpose
    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240408-plasmid-map-sequence-id-478118

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  15. pETDuet-solo-NHis6-NocO-H279A

    Plasmid
    #240406
    Purpose
    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240406-plasmid-map-sequence-id-478117

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  16. pETDuet-solo-NHis6-NocO-E94A

    Plasmid
    #240401
    Purpose
    expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240401-plasmid-map-sequence-id-478116

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  17. pQE30-HypocratesCS

    Plasmid
    #161957
    Purpose
    Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    HypocratesCS
    Use
    Tags
    6xHIs tag
    Expression
    Bacterial
    Mutation
    changed cysteine 355 to serine
    Promoter
    T5
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161957-plasmid-map-sequence-id-324311

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  18. pCS2+-HypocratesCS

    Plasmid
    #161960
    Purpose
    Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives
    Depositor
    Dmitry Bilan
    Article
    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:
    Insert
    Hypocrates
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Cysteine 355 to Serine
    Promoter
    CMV, SP6
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161960-plasmid-map-sequence-id-324303

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Showing: 1101 - 1120 of 1378 results