We narrowed to 144,455 results for: ins

1. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa

   Plasmid

   #221350

   Purpose

   Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa

   Plasmid

   #221362

   Purpose

   Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa; to be used with Anti-ProC [IPI-HPC-4] heavy chain (Plasmid 221361) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGS_N_3xFLAG

   Plasmid

   #101654

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, 3xFLAG tag
   

   Use

   Tags

   3xFLAG tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7-TaGu-RTD-3xF-ORF

   Plasmid

   #238490

   Purpose

   For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   DD660/661->AA

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7-TaGu-END-3xF-ORF

   Plasmid

   #238489

   Purpose

   For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   DD1057/1070->AA

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa

   Plasmid

   #221354

   Purpose

   Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGS_C_3xFLAG

   Plasmid

   #101653

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, 3xFLAG tag
   

   Use

   Tags

   3xFLAG tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUb-G76V-GFP-IRES-DsRed

   Plasmid

   #225503

   Purpose

   Expression of Ub-G76V-GFP along dsRED from one mRNA

   Depositor

   David Rubinsztein

   Article

   Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi:

   Insert

   Ub-G76V-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Promoter

   CMV promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-SdrG-B2-ELP-HIS-ybbR

   Plasmid

   #169134

   Purpose

   E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid

    #169133

    Purpose

    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Use

    Tags

    3xELP linker, 6x Histag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGS_N_Strep

    Plasmid

    #101651

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, Twin-Strep tag
    

    Use

    Tags

    Twin-Strep tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGS_C_Strep

    Plasmid

    #101652

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, Twin-Strep tag
    

    Use

    Tags

    Twin-Strep tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SBC009876

    Plasmid

    #226283

    Purpose

    Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

    Depositor

    Nigel Scrutton

    Article

    Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ApoE3_mCh-SspB (pBS1144)

    Plasmid

    #185326

    Purpose

    For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ApoE2_mCh-SspB (pBS1142)

    Plasmid

    #185324

    Purpose

    For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hTln1-R1R2-17b

    Plasmid

    #191427

    Purpose

    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

    Depositor

    Ben Goult

    Article

    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

    Insert

    Human Talin1 R1R2 domains (TLN1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    contains 17AA insertion from inclusion of exon 17b

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid

    #185299

    Purpose

    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid

    #185298

    Purpose

    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid

    #185294

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D125I

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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