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pEY37

Plasmid

#191041

Purpose

srsx-3 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srsx-3 (srsx-3 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY35

Plasmid

#191039

Purpose

srh-79 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srh-79 (srh-79 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY45

Plasmid

#191049

Purpose

gcy-35 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-35 (gcy-35 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR-221-P3-P5

Plasmid

#186350

Purpose

Gateway donor vector with AttP3 AttP5 recombination sites

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC005

Plasmid

#171140

Purpose

For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

XylS-Pm

Use

CRISPR

Expression

Bacterial

Promoter

Pm

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG418R-GMRWhite

Plasmid

#165883

Purpose

G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p3xP3-Hsp70b

Plasmid

#165773

Purpose

HSP70B Promoter fused to synthetic 3xP3 eye driver

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3x3-Hsp70b

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pED17x11

Plasmid

#134814

Purpose

sfGFP expression plasmid for characterizing qtRNA charging

Depositor

Ahmed Badran

Publication

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

sfGFP-151-TAGA

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

151-TAGA

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTJV1ScKm-rpoB

Plasmid

#166980

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

Depositor

Christopher Reisch

Publication

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

NeoR/KanR

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Publication

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHygroR-GMRWhite

Plasmid

#165886

Purpose

Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

Plasmid

#137859

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Expression

Mammalian

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Publication

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRVV655

Plasmid

#119019

Purpose

Drives TP901-1 recombinase expression in the D. melanogaster germ line

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 recombinase

Tags

SV40 NLS

Expression

Insect

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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OA959C

Plasmid

#104968

Purpose

Expresses anti-ZIKV transgene in Ae. aegypti

Depositor

Omar Akbari

Publication

Buchman et al Proc Natl Acad Sci U S A. 2019 Feb 5. pi

Insert

Anti-Zika Transgene

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Publication

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Expression

Mammalian

Promoter

U6

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pIM35

Plasmid

#74644

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection marker

Depositor

Jiří Hašek

Publication

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12m

Plasmid

#69943

Purpose

pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Publication

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

pSCKtheoRaj12m

Use

Synthetic Biology; Psc101 origin of replication

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMM750

Plasmid

#68899

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMM732

Plasmid

#68897

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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