We narrowed to 16,212 results for: NSI;

Showing: 11221 - 11240 of 16212 results

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pcDNA3.1-AtSLAC1 6D

Plasmid

#212947

Purpose

Vector used for expression of AtSLAC1 6D in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Expression

Mammalian

Mutation

T62D/S65D/S107D/S124D/S146D/S152D

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA D34S

Plasmid

#210813

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA K230A

Plasmid

#210811

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA D34S

Plasmid

#210807

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_6F→A

Plasmid

#194269

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

F276A, F283A, F289A, F367A, F397A, F401A

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→A_CR

Plasmid

#194263

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

M322A, M323A, M336A, M337A, M339A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→A_IDR

Plasmid

#194264

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

M307A, M311A, M359A, M405A, M414A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→L_IDR

Plasmid

#194265

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307L, M311L, M359L, M405L, M414L mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5M→Y_IDR

Plasmid

#194266

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

M307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_5A→M_IDR

Plasmid

#194267

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

A297M, A367M, A381M, A388M, A391M mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CRF0.5

Plasmid

#208657

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-tagged Sox17FNV

Plasmid

#206391

Purpose

Expresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Sox17FNV (Sox17 Mouse)

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mBrn2-3xflag

Plasmid

#206390

Purpose

Expresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW2-NgBR R290H

Plasmid

#203167

Purpose

Yeast expression vector for hNUS1 R290H

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

NUS1 (NUS1 Human)

Expression

Yeast

Mutation

R290H

Promoter

TDH3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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mEos3.2trLATm2allL+A

Plasmid

#203750

Purpose

Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATm2(allL+A) (LAT Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-CHMP2A-myc, L216D/L219D

Plasmid

#180645

Purpose

Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP2A (CHMP2A Human)

Tags

Myc

Expression

Mammalian

Mutation

L216D, L219D

Promoter

pCAG

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNA1 MIT residues 1-79, V55D

Plasmid

#180615

Purpose

Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-79, V55D mutation

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 R14A

Plasmid

#160052

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

R14A mutation

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 V55D

Plasmid

#160053

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

V55D mutation

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-π-EB1_IRES-mCherry

Plasmid

#197427

Purpose

Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)

Use

CRISPR and Synthetic Biology

Tags

GCN4 leucine zipper, LOV2, Zdk1, and mCherry

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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