We narrowed to 14,341 results for: cas9 genes

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pRExT2A-NT-BTy

Plasmid

#232206

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PTy

Plasmid

#232203

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmSc

Plasmid

#232208

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BTy

Plasmid

#232209

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PTy

Plasmid

#232202

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PmSc

Plasmid

#232548

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarlet

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Mutation

None

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pG3H-PE3max-attR1R2

Plasmid

#213049

Purpose

Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Inserts

Cas9 nickase
M-MLV Reverse Transcriptase

Use

Note: this plasmid needs helper pvs1-vir2 for rep…

Expression

Plant

Mutation

H840A, R240K, N413K,

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgHK2_2

Plasmid

#163461

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLY152

Plasmid

#130950

Purpose

dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Inserts

dcas9
tetR
sfgfp

Use

Synthetic Biology

Tags

ASV tag

Expression

Bacterial

Mutation

Mutations D10A and H840A on WT cas9. Synonymous m…

Promoter

PpspA-2G6 and Ptet

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA giantin/GOLGB1

Plasmid

#222315

Purpose

CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

gRNA targeting GOLB1 (GOLGB1 Human)

Use

CRISPR

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pUt-mNF-Cas13d

Plasmid

#224789

Purpose

LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

mCherry-P2A-RfxCas13d

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV-d2i

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pTij_sg_mmu_Med6_C_terminal

Plasmid

#197869

Purpose

CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti NG-ABE8e_P2A_GFP + PuroR

Plasmid

#242000

Purpose

Lentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.

Depositor

Frank Buchholz

Article

Wang et al Genome Biol. 2025 Jul 22;26(1):217. doi:

Insert

NG-ABE8e

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDGE347

Plasmid

#153228

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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