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pGL3-noSV40-EC1.45-human_min1-coelacanth_min2

Plasmid

#173971

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and coelacanth minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-chicken_min2

Plasmid

#173968

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and chicken minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-chicken_min2

Plasmid

#173968

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and chicken minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#7

Plasmid

#173991

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #7 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#7

Plasmid

#173991

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #7 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-platypus_min2

Plasmid

#173967

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-EC1.45-human_min1-platypus_min2

Plasmid

#173967

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7

Plasmid

#173993

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated

Use

Luciferase

Mutation

3X Coordinator motif mutation #5-6-7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7

Plasmid

#173993

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated

Use

Luciferase

Mutation

3X Coordinator motif mutation #5-6-7

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant

Plasmid

#173964

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs

Use

Luciferase

Mutation

3 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant

Plasmid

#173964

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs

Use

Luciferase

Mutation

3 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-mouse_min2

Plasmid

#173965

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-EC1.45-human_min1-mouse_min2

Plasmid

#173965

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-lizard_min2

Plasmid

#173969

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-EC1.45-human_min1-lizard_min2

Plasmid

#173969

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-RPS14(WT)-Myc

Plasmid

#122236

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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