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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,870 results for: ina

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Showing: 11241 - 11260 of 24870 results

  1. Halo-Tev-Akt3 WT-2X Flag

    Plasmid
    #87607
    Purpose
    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein
    Depositor
    Yimon Aye
    Insert
    Akt3 isoform 2 (AKT3 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    2 tandem flag tags and His-Halo-Tev
    Expression
    Mammalian
    Mutation
    Akt3 isoform 2
    Promoter
    SP6
    Available Since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87607-plasmid-map-sequence-id-197919

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  4. pGEX-2T-FRB-W2027F

    Plasmid
    #73341
    Purpose
    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; W2027F
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pIS1 mIL6R

    Plasmid
    #60797
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60797-plasmid-map-sequence-id-97808

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  10. MSP10-bio

    Plasmid
    #47719
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP10
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47719-plasmid-map-sequence-id-68458

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  11. ASP-bio

    Plasmid
    #47745
    Purpose
    Expresses enzymatically monobiotinylated full-length ASP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised ASP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47745-plasmid-map-sequence-id-68757

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  12. RH1-bio

    Plasmid
    #47748
    Purpose
    Expresses enzymatically monobiotinylated partial RH1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH1 (partial)
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47748-plasmid-map-sequence-id-68773

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  13. RAP1-bio

    Plasmid
    #47794
    Purpose
    Expresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RAP1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47794-plasmid-map-sequence-id-68800

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Showing: 11241 - 11260 of 24870 results