We narrowed to 24,453 results for: nes
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Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-H1-SGIPZ_NF1 sh1miR
Plasmid#119206Purposeknockdown of NF1DepositorAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG mouse Bmf
Plasmid#17243DepositorAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
AiP14478 - pAAV-AiE0456m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4478)
Plasmid#229923PurposeAiE0456m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell typesDepositorInsertSYFP2
UseAAVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)
Plasmid#224016PurposeAiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neuronsDepositorInsertSYFP2
UseAAVMutationNAPromoterminBGAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)
Plasmid#224536PurposeA Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3DepositorInsertIRES-FLAG- NLS-mScarlet-I3
UseGateway CloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-vp16
Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
TagsHis and VP16 AD (413-490)ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205097Purposefusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogeneticsDepositorInsertmNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1
UseAAVPromoterhSynAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E)dTomato
Plasmid#200110PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
ExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element, no other promoter elemen…Available SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shSLC2A1-1
Plasmid#193702PurposeConstitutive lentiviral expression of SLC2A1 shRNADepositorInsertSLC2A1 (SLC2A1 Human)
UseLentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shSLC2A1-2
Plasmid#193703PurposeConstitutive lentiviral expression of SLC2A1 shRNADepositorInsertSLC2A1 (SLC2A1 Human)
UseLentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCrebbp#2/Cre
Plasmid#193210PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp geneDepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
ExpressionMammalianPromoterEF-1a promoter and RPBSA promoterAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_NAP1L1-Halo
Plasmid#175342PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode
Plasmid#170229PurposeBarcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-METAP1-3xFLAG
Plasmid#163453Purposelentiviral overexpression vector for human METAP1DepositorInsertMETAP1 (METAP1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable SinceMarch 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]
Plasmid#159796PurposeHistamine based negative selection marker to paralyze animals with arraysDepositorInsertpEXP[Prpl-3 | histamine | rpl-3 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF64-2xNSTREP
Plasmid#136224PurposeExpresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64DepositorInsertORF64
ExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF26-2xCSTREP
Plasmid#136186PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26DepositorInsertORF26
ExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only