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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,472 results for: sequence

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Showing: 11241 - 11260 of 13472 results

  3. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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  6. Cer(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #55708
    Purpose
    An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-beta5 (GNB5 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-t…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55708-plasmid-map-sequence-id-114838

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  10. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  17. pGAPTrap-IRESMuro

    Plasmid
    #82336
    Purpose
    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    IRESMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    82336-plasmid-map-sequence-id-155824

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Showing: 11241 - 11260 of 13472 results