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pJK225

Plasmid

#71124

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2_TagBFP

Plasmid

#83031

Purpose

Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry+full length_TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

3 mutations in sfCherry full length: E118Q, T128I…

Promoter

SFFV

Available Since

Jan. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-C1

Plasmid

#84355

Purpose

Expresses mCyRFP1 fluorescent protein in an empty backbone

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1

Expression

Mammalian

Mutation

A161K and N41A, reletive to CyRFP1

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_HuD

Plasmid

#65760

Purpose

constitutively expresses FLAG-HA-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pHJ322

Plasmid

#20677

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 558 to Asp

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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Hox A11a

Plasmid

#36462

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11a (hoxa11a Zebrafish)

Use

Riboprobe synthesis

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
CIp10 - ffDronpa ATGme

Plasmid

#163118

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

ffDronpa ATGme

Use

Synthetic Biology; Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-tdTomato-polyA

Plasmid

#174022

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

tdTomato

Use

Zebrafish knock-in tagging

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN4 WT

Plasmid

#59320

Purpose

Expression plasmid of HA-tagged wildtype human Neuroligin 4

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN4 (NLGN4X Human)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Talin

Plasmid

#80026

Purpose

Mammalian expression of mCherry-Talin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

Talin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_psychLight2

Plasmid

#163910

Purpose

Expresses psychLight2 in mammalian cells

Depositor

Lin Tian

Article

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHen2 Avi-tag-linker-p3

Plasmid

#119819

Purpose

For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

linker with Avi-tag and p3

Tags

Avi-tag

Expression

Bacterial

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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p4458 FLAG-hPLIC-1

Plasmid

#8663

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-1 (UBQLN1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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