We narrowed to 18,716 results for: VARS

1. DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

   Plasmid

   #185296

   Purpose

   For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DSUP_RAMVA_1-208
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCapVQ329R

   Plasmid

   #183029

   Purpose

   the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28

   Depositor

   Ute Romling

   Article

   Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

   Insert

   patatin-like phospholipase variant CapVQ329R
   

   Tags

   No

   Expression

   Bacterial

   Mutation

   glutamine 329 changed to arginine

   Promoter

   pBAD promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-SopB(K525A)

   Plasmid

   #183659

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K525A

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-SopB(K528A)

   Plasmid

   #183660

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K528A

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-SopB(33-554)

   Plasmid

   #183661

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-554

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-SopB(33-520)

   Plasmid

   #183662

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-520

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-SopB(68-554)

   Plasmid

   #183663

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 68-554

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SopB(C460S)-EGFP

   Plasmid

   #183656

   Purpose

   C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-SopB(C460S)

   Plasmid

   #183658

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV_EF1a_MAPK9-P2A-Hygro_Barcode

    Plasmid

    #170238

    Purpose

    Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAPK9 (MAPK9 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

    Plasmid

    #160583

    Purpose

    Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    YFP
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. human TLNRD1-4H pET151

    Plasmid

    #159386

    Purpose

    Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
    

    Tags

    his-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    TLNRD1 4-helix domain (residues 143-273)

    Promoter

    T7

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

    Plasmid

    #160617

    Purpose

    TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    mini35s

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

    Plasmid

    #160582

    Purpose

    35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    attR:P35S:TMtb:attL
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD 5'UTR:YFP:Tnos (GB1483)

    Plasmid

    #160557

    Purpose

    Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    5'UTR:YFP:Tnos
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP N1 AURKA deltaNter

    Plasmid

    #157753

    Purpose

    Expression of AURKA without Nter, fused to GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)

    Plasmid

    #160581

    Purpose

    Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminator

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    Luc
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

    Plasmid

    #160614

    Purpose

    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    mini35s

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

    Plasmid

    #160615

    Purpose

    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    mini35s

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA BRCA2 S3070F

    Plasmid

    #139325

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 S3070F using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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