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pRK5_mEGFP-PAX-FOXO1

Plasmid

#237643

Purpose

For overexpression of mEGFP-PAX-FOXO1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

PAX-FOXO1 (FOXO1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 1

Plasmid

#237649

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 1 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 2

Plasmid

#237650

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 2 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 10

Plasmid

#237658

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 10

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 10 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS926

Plasmid

#228237

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mCherry
Beta globin

Tags

3xFLAG, HA, and PuroR

Expression

Mammalian

Mutation

PTC39

Promoter

TRE3GV and none

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS927

Plasmid

#228238

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mCherry
Beta globin

Tags

3xFLAG, HA, and PuroR

Expression

Mammalian

Promoter

TRE3GV and none

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.8D2Q.Tau2N4R

Plasmid

#226400

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.20D3Q.Tau2N4R

Plasmid

#226401

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Mutation

20D3Q, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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CIRC#del

Plasmid

#186344

Purpose

Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pQCX-CD28 Y189F

Plasmid

#223641

Purpose

Retroviral expression of mouse CD28 (Y189F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (Cd28 Mouse)

Use

Retroviral; mammalian expression

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-mTFP1

Plasmid

#223638

Purpose

Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(CD45TM)

Plasmid

#223629

Purpose

Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(Y191F)

Plasmid

#223628

Purpose

Lentiviral expression of human CD28 (Y191F) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y209F)

Plasmid

#223627

Purpose

Lentiviral expression of human CD28 (Y209F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y209F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(CD45TM)

Plasmid

#223626

Purpose

Lentiviral expression of human CD28 (CD45TM) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y191F)

Plasmid

#223625

Purpose

Lentiviral expression of human CD28 (Y191F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(CD45TM)

Plasmid

#223624

Purpose

Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(Y191F)

Plasmid

#223623

Purpose

Lentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-PDL1-LgBiT

Plasmid

#223619

Purpose

Lentiviral expression of human PD-L1 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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