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AAV2/9-shMBVR-HO1-Fd-Fnr-CW3SL

Plasmid

#184003

Purpose

AAV production

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

shMBVR-HO1-Fd-Fnr-CW3SL

Use

AAV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK037

Plasmid

#180829

Purpose

T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

CnGdmB (CNE_RS35790 Cupriavidus necator N-1)

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-ChroMEd15-mTFP

Plasmid

#184240

Purpose

channelrhodopsin ChroME fused to mTFP

Depositor

Yiyang Gong

Article

Beck et al Biophys J. 2022 Mar 21. pii: S0006-3495(

Insert

ChroMEd15-mTFP

Use

Lentiviral

Tags

mTFP1

Expression

Mammalian

Mutation

deleted ChroME amino acids 317-331

Promoter

Camk2

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0180

Plasmid

#172814

Purpose

Level 1 module containing TP-CFP_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-CFP-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0071

Plasmid

#172785

Purpose

Level 0 module containing CT_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer+NtVenus 1-210 STOP pAGM1301 (CT)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_045

Plasmid

#180543

Purpose

Entry vector containing RBS 30,000 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 30,000 a.u. designed strength

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_046

Plasmid

#180544

Purpose

Entry vector containing RBS 250 a.u. designed strength

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sRBS 250 a.u. designed strength

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0867

Plasmid

#177085

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0861

Plasmid

#177078

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0705

Plasmid

#177035

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0059

Plasmid

#177023

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10

Use

Synthetic Biology

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzO139

Plasmid

#172970

Purpose

pWSK29 with Vibrio cholerae wzzO139 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzz gene from Vibrio cholerae O139

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKMyc-fly DGCR8

Plasmid

#171561

Purpose

Encodes fly DGCR8 protein

Depositor

Yan Zeng

Insert

Pasha(CG1800) (pasha Fly)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-His-GBAi

Plasmid

#171755

Purpose

For the expression of His-GBAi in bacteria

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

His

Expression

Bacterial

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ282 (TsCas12a)

Plasmid

#138114

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

TsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ283 (MlCas12a)

Plasmid

#138115

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MlCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ287 (BsCas12a)

Plasmid

#138123

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc259

Plasmid

#161951

Purpose

Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter

Expression

Bacterial

Promoter

PTet*

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D977A

Plasmid

#129674

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D977A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-E848A

Plasmid

#129675

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Tags

E848A; AacCas12b is rice codon optimized

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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