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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28,504 results for: nes

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Showing: 11361 - 11380 of 28504 results

  3. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Publication
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Tags
    His Tag
    Expression
    Bacterial
    Promoter
    T7lac promoter
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

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  4. mCh-KIF17*-strep

    Plasmid
    #120167
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 17 (KIF17 Human)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF17: 1-509 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120167-plasmid-map-sequence-id-247505

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  6. pGFP-Kif5cmut

    Plasmid
    #71854
    Purpose
    mammalian expression of mutant GFP-Kif5c
    Depositor
    Michelle Peckham
    Publication
    Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kif5c (Kif5c Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    T93N mutation and also a novel SpeI site (1652). …
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  12. pMaCTag-P15

    Plasmid
    #120026
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miRFP670
    Use
    Pcr template
    Tags
    miRFP670
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120026-plasmid-map-sequence-id-232633

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  16. WDR5 (2GNQ)

    Plasmid
    #25489
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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Showing: 11361 - 11380 of 28504 results