We narrowed to 4,411 results for: Ado;

1. T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)

   Plasmid

   #216717

   Purpose

   Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   HaloTag-RGD-PILATeS
   

   Use

   Tags

   6xHis and HaloTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-LacO-Halo-PILATeS-RGD (700pM)

   Plasmid

   #216714

   Purpose

   Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   HaloTag-PILATeS-RGD
   

   Use

   Tags

   6xHis and HaloTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)

   Plasmid

   #216715

   Purpose

   Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   HaloTag-RGD-PILATeS
   

   Use

   Tags

   6xHis and HaloTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF1b-His8-TEV-PI31-1-151

   Plasmid

   #202593

   Purpose

   Bacterial expression of HsPI31-1-151

   Depositor

   Brenda Schulman

   Article

   Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

   Insert

   PI31 (PSMF1 Human)
   

   Use

   Tags

   8xHis, TEV

   Expression

   Bacterial

   Mutation

   aa 1-151 only

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cut190*_pET21b

   Plasmid

   #215404

   Purpose

   Expression construct for Cut190* gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Cut190*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S226P/R228S

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pToxEXPr6

   Plasmid

   #218290

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJQR3

   Plasmid

   #218289

   Purpose

   Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. RoL10X_pET29b

   Plasmid

   #215427

   Purpose

   Expression construct for RoL10X gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   RoL10X
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. NylB-SCY_pET29b

   Plasmid

   #215415

   Purpose

   Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB-SCY
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R187S/F264C/D370Y

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJET-LB-geneticin

    Plasmid

    #213468

    Purpose

    Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Left border of the TSI locus 1-split fragment of geneticin cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2x3APWWP-IFP2.0C

    Plasmid

    #215752

    Purpose

    Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2x3APWWP-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2xCBX7CD-IFP2.0C

    Plasmid

    #215754

    Purpose

    Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xCBX7CD-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK2 RPS28 UTR1 SCR

    Plasmid

    #203158

    Purpose

    RPS28 UTR luciferase assay plasmid (SCR UTR1, sites 1 and 2)

    Depositor

    Susan Baserga

    Article

    Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

    Insert

    RPS28 UTR (RPS28 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    scrambled the nucleotides in the two MIR-28 bindi…

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Combined γB2FL-FRET probe

    Plasmid

    #209094

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Combined γB2ΔICD-FRET probe

    Plasmid

    #209093

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNHT7

    Plasmid

    #186753

    Purpose

    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning backbone

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRBad-Z-C-DiB-RM

    Plasmid

    #168477

    Purpose

    C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    Leucine Zipper + C-fragment of DiB-RM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET11a-Z-N-DiB-RM

    Plasmid

    #168475

    Purpose

    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    N-fragment of DiB-RM + Leucine Zipper
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFETCh_ELK1_iso1

    Plasmid

    #135737

    Purpose

    Donor vector for 3' FLAG tag of human ELK1_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ELK1_iso1 Homology arms (ELK1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFETCh_MAF1_iso1

    Plasmid

    #135732

    Purpose

    Donor vector for 3' FLAG tag of human MAF1_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MAF1_iso1 Homology arms (MAF1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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