We narrowed to 2,548 results for: EFS

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pRDA_103

Plasmid

#133460

Purpose

H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFC482

Plasmid

#246439

Purpose

The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

CasE

Tags

NLS

Expression

Mammalian

Mutation

SapI domesticated

Promoter

EF-1a

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-P49

Plasmid

#202176

Purpose

Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-glna

Plasmid

#202183

Purpose

Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

glna terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_tr

Plasmid

#202171

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 truncated terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-epyC

Plasmid

#202185

Purpose

Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

epyC terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-acc2

Plasmid

#202184

Purpose

Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

acc2 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-dph1

Plasmid

#202182

Purpose

Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

dph1 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-NR

Plasmid

#202179

Purpose

Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-fcpB

Plasmid

#202177

Purpose

Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fcpB terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-GPI1

Plasmid

#202175

Purpose

Level 0 plasmid containing a terminator sequence from gene 23924 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

GPI1 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-vapol

Plasmid

#202174

Purpose

Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-lysA

Plasmid

#202173

Purpose

Level 0 plasmid containing a terminator sequence from gene 21592 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

lysA terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-HO

Plasmid

#202170

Purpose

Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-NRT

Plasmid

#202169

Purpose

Level 0 plasmid containing a terminator sequence from gene 54101 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NRT terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-sit4

Plasmid

#202168

Purpose

Level 0 plasmid containing a terminator sequence from gene 48707 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sit4 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-CaMK2

Plasmid

#202167

Purpose

Level 0 plasmid containing a terminator sequence from gene 39236 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CaMK2 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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