Addgene

We narrowed to 18,785 results for: INO

Showing: 1121 - 1140 of 18785 results

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Use

Tags

5HT6 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-myc-USP10

Plasmid

#184034

Purpose

Expresses myc-USP10 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

Insert

USP10 (USP10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMVf

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI4

Plasmid

#42528

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI4 (WDR45 Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
CFP-FKBP

Plasmid

#162433

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

CFP-FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTomatoSlicer

Plasmid

#221085

Purpose

Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKm-HCBE

Plasmid

#170333

Purpose

a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized CBEs

Use

CRISPR

Tags

Expression

Mutation

Cas9n-D10A

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKm-HGBE

Plasmid

#170334

Purpose

a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized GBEs

Use

CRISPR

Tags

Expression

Mutation

Cas9n-D10A

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Tags

Expression

Mutation

Cas9n-D10A

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tom20-mCh-FKBP

Plasmid

#162436

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Tom20-mCh-FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Uch37C88A

Plasmid

#19416

Depositor

Ronald Conaway, Joan Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Use

Tags

2XHA

Expression

Mammalian

Mutation

C88A

Promoter

Available since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCUPI NPD-GFP

Plasmid

#1191

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 (SUP35 Budding Yeast)

Use

Cup1 promoter

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pDB038a

Plasmid

#99211

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

amber codons at positions 29 and 184 of gIII

Promoter

Ppsp and PproK

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRF0G-Tyr

Plasmid

#113644

Purpose

Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

MjYRS and tRNA gene

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDB038b

Plasmid

#99212

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

amber codons at positions 29, 83, and 184 of gIII

Promoter

Ppsp and PproK

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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CF-PIPKi

Plasmid

#20158

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)

Use

Tags

Expression

Mammalian

Mutation

D253A mutation in PIPK. C-terminus (amino acids …

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-GID1

Plasmid

#37306

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GID1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pEF6 Vps53-V5-6His

Plasmid

#175097

Purpose

V5 tagged Vps53 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps53 (Vps53 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CF-PIPK

Plasmid

#20157

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-PIPK (Pip5k1c Mouse)

Use

Tags

Expression

Mammalian

Mutation

C-terminus (amino acids 636-661)truncated, R445E,…

Promoter

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps54-V5-6His

Plasmid

#175098

Purpose

V5 tagged Vps54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps54 (Vps54 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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