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pXPR_003 sgAP2M1 guide 1

Plasmid

#193600

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 1 (AP2M1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 1

Plasmid

#193591

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 1 (RELA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 1

Plasmid

#193593

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 1 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 2

Plasmid

#193594

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 2 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide-PolB1-puro

Plasmid

#177146

Purpose

Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolBgRNA1 (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-PML peptide

Plasmid

#175246

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 ZFAND3-guide4

Plasmid

#125516

Purpose

CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 EGFP-guide2

Plasmid

#118167

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 PSMB5 guide 2

Plasmid

#117074

Purpose

single guide RNA targeting PSMB5; guide 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

LMPX (PSMB5 Human)

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Mutation

s1006-gggs-d1221 deletion

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Promoter

CAG

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CRY2-TSS-guide3

Plasmid

#125491

Purpose

CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CRY2-TSS-guide2

Plasmid

#125490

Purpose

CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-polg guide

Plasmid

#107598

Purpose

An entry vector with U6a promoter driving polg guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

polg guide RNA sequence

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide Cyclone-Puro P2A EGFP

Plasmid

#247497

Purpose

Acyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion protein

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

puromycin n-acetyltransferase with Cyclone insertion

Use

Lentiviral and Synthetic Biology

Tags

Puro(Cyclone)-P2A-EGFP

Expression

Mammalian

Mutation

Cyclone was inserted after Glutamine135 of puromy…

Promoter

EF-1a

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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