We narrowed to 11,481 results for: NSI

1. Bxb1-GA Donor_hEF1a

   Plasmid

   #229779

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvoltac-EcW-TGA-h14

   Plasmid

   #218764

   Purpose

   encodes EcWRS-h14 and EcW-tRNA-TGA

   Depositor

   Abhishek Chatterjee

   Article

   Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

   Insert

   EcWRS-h14
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S8A, V144G, V146C

   Promoter

   tacI

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC-1-BeYDV-LS-L-11

   Plasmid

   #197717

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   BeYDV LSL in c-sense with GG expansion to v-sense ORF position
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-1-BeYDV-LRS-L-11

   Plasmid

   #197718

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   BeYDV LRSL in c-sense with GG expansion to v-sense ORF position
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYSD085

   Plasmid

   #212922

   Purpose

   Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   649 Stalk
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIVT-PuroR

   Plasmid

   #232753

   Purpose

   IVT template for making modRNA encoding the Puromycin resistance gene

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   PuroR
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXJ-His-PDCD1LG2

   Plasmid

   #232660

   Purpose

   transient expresses PD-L2 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   PDCD1LG2 (PDCD1LG2 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA Donor_CAG

   Plasmid

   #229780

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-O-GECO1

   Plasmid

   #46025

   Purpose

   Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   O-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pSF-CjPglB-LpxE

    Plasmid

    #128389

    Purpose

    Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Inserts

    PglB oligosaccharylktransferase
    LpxE phosphatase
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and pBAD

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5T7-LYCipi-ggpps

    Plasmid

    #122017

    Purpose

    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Insert

    GGPP synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized, truncated first 98 amino acids, …

    Promoter

    T7

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUE1-TSP-BFLF1

    Plasmid

    #162657

    Purpose

    Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC-1-WDV-LRS-L-11

    Plasmid

    #197720

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    WDV LRSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCiVSP(C363S)-SD64TF

    Plasmid

    #80334

    Purpose

    Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

    Depositor

    Yasushi Okamura

    Article

    Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

    Insert

    Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Changed Cysteine 363 to Serine

    Promoter

    SP6

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMZ-176

    Plasmid

    #195424

    Purpose

    Transient, constitutive expression of synZiFTR: ZF1-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC Ad VAI

    Plasmid

    #175518

    Purpose

    For mammalian expression of adenovirus VAI RNA

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    Ad VAI RNA
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL-VinculinTS-I997A

    Plasmid

    #111832

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A) (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed vinculin isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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