We narrowed to 21,207 results for: NUC

1. pDZ585 pKAN 2x-mCherry

   Plasmid

   #72236

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   2x-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-RCC1-polyA

   Plasmid

   #59762

   Purpose

   Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   RCC1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-Cer.zf1

   Plasmid

   #61389

   Purpose

   mRNA synthesis of cerulean codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cer.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-2A-TAL6-MSTN

   Plasmid

   #72597

   Purpose

   MSTN TALEN with 2A-GFP tagged

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   TALEN for MSTN
   

   Use

   TALEN

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKJ237

   Plasmid

   #212320

   Purpose

   Express KnFnuc

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   KnFnuc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKJ226

   Plasmid

   #212316

   Purpose

   Express DpFnuc with pureexpress

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   DpFnuc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Banshee-Sh-Ctrl

   Plasmid

   #85587

   Purpose

   Control hairpin sequence used in gene knockdown experiements

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   sh-Ctrl
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-FLAG-SENP3-C526A

   Plasmid

   #115522

   Purpose

   Mammalian expression construct

   Depositor

   Martine Roussel

   Article

   Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

   Insert

   SENP3-C526A (Senp3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C526A

   Promoter

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/HisC 14-3-3 epsilon K49E

   Plasmid

   #11943

   Depositor

   Michael Yaffe

   Article

   Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

   Insert

   Ywhae (Ywhae Rat)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   K49E

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pDZ514 pURA MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72230

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

    Plasmid

    #72234

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADE3p

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTAL3

    Plasmid

    #31034

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    AcV5 and FokI nuclease

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. GPKAnls

    Plasmid

    #61094

    Purpose

    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal

    Depositor

    Steven Green

    Article

    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

    Insert

    pkac2
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. GPKAnes

    Plasmid

    #61092

    Purpose

    GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal

    Depositor

    Steven Green

    Article

    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

    Insert

    pkac2
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pTAL4

    Plasmid

    #31035

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    AcV5 and FokI nuclease

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-HIRAmut-polyA

    Plasmid

    #59782

    Purpose

    This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change from 'GATCGAAGTTTG' to 'GAc…

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK571

    Plasmid

    #75379

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Gene synthesis

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glycine-209 to aspartate and synthetic ge…

    Promoter

    n/a

    Available since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72233

    Purpose

    Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac WT SOX

    Plasmid

    #192055

    Purpose

    Expression vector for WT SOX

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    WT SOX (ORF37)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-28a(+)-T7-OmpA-MNaseB-6xHis

    Plasmid

    #214809

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease B
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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