We narrowed to 25,057 results for: Nes

1. pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

   Plasmid

   #193852

   Purpose

   Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   Guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV PIG IMP-1-short

   Plasmid

   #21659

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
   

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFW5000

   Plasmid

   #224214

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMVV205

   Plasmid

   #215342

   Purpose

   Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRII p15

   Plasmid

   #16454

   Depositor

   Bert Vogelstein

   Article

   Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

   Insert

   p15 (CDKN2B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAG_mCh_p85a_mCh

   Plasmid

   #199630

   Purpose

   FRET acceptor for IGF1R sensor

   Depositor

   Ryohei Yasuda

   Article

   Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

   Insert

   p85a-SH2 (Pik3r1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mNeonGreen-IETD-NanoLuc

   Plasmid

   #98288

   Purpose

   caspase-8 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS NL4-3 envFS eGFP PR-D25A

    Plasmid

    #101334

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Protease D25A

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSC12

    Plasmid

    #104786

    Purpose

    pNB184-Cas9 entry cassette

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Fragment 1 Null

    Plasmid

    #198729

    Purpose

    Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    gRNA expression cassette in pENTR4
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET22b-His6-UBCH7

    Plasmid

    #212711

    Purpose

    Bacterial expression of His6-UBCH7

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    UBCH7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMOD_C3003

    Plasmid

    #91095

    Purpose

    Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH6HTC_IGF2BP3-Halo

    Plasmid

    #175310

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP3 (IGF2BP3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPyCAG-tagBFP-3xNLS-IRES-Hph

    Plasmid

    #183608

    Purpose

    Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    tagBFP-3xNLS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-EGFP-C-IFT70B

    Plasmid

    #218729

    Purpose

    Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

    Insert

    IFT70B (TTC30B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBbi-TIR1-tTA/Pur

    Plasmid

    #171683

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Inserts

    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter and RPBSA promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSV-PGC1

    Plasmid

    #3

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2006

    Availability

    Academic Institutions and Nonprofits only